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Cholesterol is essential for the structure and derivative
of invaginated caveolae and clathrin-coated pits, including the caveolae-dependent
endocytosis and clathrin-dependent endocytosis.
The role of cholesterol in caveolae-dependent and clathrin-dependent
endocytosis can be investigated by using methyl beta cyclodextrin (M?CD) to abate
cholesterol from the plasma membrane.
Synthesis and intake cholesterol
is required in the membrane of mammalian cells against perpendicular cellular
function, and is either synthesized in the endoplasmic reticulum, or derived
from the diet, in which case it is delivered by the bloodstream in low-density
lipoproteins.
These are taken into the cell by LDL receptor-mediated endocytosis
in clathrin-coated pits, and then hydrolysed in lysosomes.
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